Sperm chromatin structure assay. Male infertility:
In our opinion, there are several reasons for the confusion regarding the clinical usefulness of sperm DNA integrity testing.
Mean DFI value was
Odds ratio was calculated using binary logistic regression.
In of female partners at least one oocyte was aspirated.
More conflicting data exist regarding the role of sperm DNA fragmentation in relation to fertilization, pre-embryo development and pregnancy outcome in in vitro mustang slut and intracytoplasmic sperm injection ICSI.
Wiley Online Library.
One of the main problems with using sperm quality as a diagnostic tool is the huge intraindividual variation reported for sperm concentration, motility and morphology.
Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays.