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Large insertions.

Large insertions. Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos.

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We suggest that 2C-HR-CRISPR is superior to random transgenesis or standard genome-editing protocols, because it ensures highly efficient insertions at endogenous loci and defined 'safe harbor' sites.

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B Enlarged view of the large inserted sequence between C and D in chromosome







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Here, we describe two-cell homologous recombination 2C-HR -CRISPR, a highly efficient gene-editing method based on introducing CRISPR reagents into embryos at the two-cell stage, which takes advantage of the open chromatin structure and the likely increase young ebony cum homologous-recombination efficiency during the long G2 phase.







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We targeted 20 endogenous genes expressed in blastocysts with fluorescent reporters and generated reporter mouse lines.







Topics covered include the latest advances in SMRT Sequencing performance for detection indian aunty sex pussy all variant types even in difficult to sequence regions of the genome, multiplexing options to increase throughput and improve efficiency, and examples of amplicon sequencing of clinically relevant targets.







We suggest that 2C-HR-CRISPR is superior to random transgenesis or standard genome-editing protocols, because it ensures highly efficient insertions at endogenous loci and defined 'safe harbor' sites.







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